名稱(chēng) | Total GIP NL-EIA |
型號 | |
更新時(shí)間 | 2023-09-25 |
特點(diǎn) | Total GIP NL-EIA背景介紹:Mercodia Total GIP NL-ELISA provides a method for the quantitative determination of total GIP in human samples.} |
產(chǎn)品展示 / PRODUCTS
- 詳細內容
品牌 | 其他品牌 | 貨號 | 10-1278-01 |
---|---|---|---|
供貨周期 | 現貨 | 應用領(lǐng)域 | 醫療衛生,化工 |
Total GIP NL-EIA背景介紹:
Mercodia Total GIP NL-ELISA provides a method for the quantitative determination of total GIP in human samples.
Total GIP NL-EIA Summary and explanation of the test
Glucose dependent insulinotropic polypeptide (GIP) is an incretin, a gut-derivedcirculating peptide hormone that potentiates glucose-dependent insulin secretionfollowing ingestion of a meal1. GIP(1-42) (active GIP) is rapidly cleaved in circulationat the N-terminus by DPP-4 to yield GIP(3-42), regarded as a non-active form ofGIP2. Because of this quick proteolytic degradation, not only intact but also total(i.e. intact plus DPP-4-metabolized) forms of GIP must be measured to study itssecretion and processing in vivo.
Total GIP NL-EIA Principle of the procedure
Mercodia Total GIP NL-ELISA is a solid phase two-site enzyme immunoassaybased on the sandwich technique, in which two monoclonal antibodies aredirected against separate antigenic determinants on the GIP molecule.GIP in the sample reacts with anti-GIP antibodies bound to microtitration wellsand peroxidase-conjugated anti-GIP antibodies in the solution. A simple washingstep removes unbound enzyme-labelled antibody. The bound conjugate isdetected by reaction with the chemiluminescent substrate. A luminescence plate reader is used to read the intensity of light generated.
產(chǎn)品搜索
聯(lián)系我們
電話(huà)/傳真:13366128764
手機:15311648455
地址:北京市海淀區廂黃旗2號樓1層4-179室